SDS-Page
NuDT14 recombinant protein
NuDT14, 1-222aa, Human, His tag, E Coli
Gene Names
NUDT14; UGPP; UGPPase
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
NuDT14; 1-222aa; Human; His tag; E Coli; uridine diphosphate glucose pyrophosphatase; uGPP; uGPPase; NuDT14 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ
Sequence Length
222
Applicable Applications for NuDT14 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for NuDT14 recombinant protein
uridine diphosphate glucose pyrophosphatase, also known as NuDT14, is a 222 amino acid cytoplasmic protein that contains one nudix hydrolase domain and belongs to the nudix hydrolase family. NuDT14 hydrolyzes ADP-ribose into ribose 5-phosphate and AMP, and uDP-glucose to glucose 1-phosphate and uMP. Existing as a homodimer, NuDT14 binds magnesium as a cofactor and is encoded by a gene located on human chromosome 14. Recombinant human NuDT14 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for NuDT14 recombinant protein
References
Yagi T., et al. (2003) Biochem J. 370:409-415; Heyen C A., et al. (2009) Biochem Biophys Res Commun. 390: 1414-1418.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26.5 kDa (245aa) confirmed by MALDI-TOF
NCBI Official Full Name
uridine diphosphate glucose pyrophosphatase isoform 1
NCBI Official Synonym Full Names
nudix hydrolase 14
NCBI Official Symbol
NUDT14
NCBI Official Synonym Symbols
UGPP; UGPPase
NCBI Protein Information
uridine diphosphate glucose pyrophosphatase
UniProt Protein Name
Uridine diphosphate glucose pyrophosphatase
Protein Family
UniProt Gene Name
NUDT14
UniProt Synonym Gene Names
UGPP; UDPG pyrophosphatase; UGPPase; Nudix motif 14
NCBI Description
The protein encoded by this gene is a member of the Nudix hydrolase family. Nudix hydrolases eliminate potentially toxic nucleotide metabolites from the cell and regulate the concentrations and availability of many different nucleotide substrates, cofactors, and signaling molecules. This enzyme contains a Nudix hydrolase domain and is a UDPG pyrophosphatase that hydrolyzes UDPG to produce glucose 1-phosphate and UMP. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Uniprot Description
Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP-mannose.
Research Articles on NuDT14
Similar Products
Product Notes
The NuDT14 nudt14 (Catalog #AAA205307) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's NuDT14 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NuDT14 nudt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ. It is sometimes possible for the material contained within the vial of "NuDT14, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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