CHST5 / GlcNAc6ST-3 recombinant protein

Mouse CHST5 / GlcNAc6ST-3 Protein (Fc Tag)

Cat. Num. AAA2546638

• Gene Names: Chst5; GST-4; Gn6st-3; AI173964; I-GlcNAc-6-ST
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: CHST5 / GlcNAc6ST-3; Mouse CHST5 / GlcNAc6ST-3 Protein (Fc Tag); Gst4; CHST5 / GlcNAc6ST-3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 395

• Application Notes: The recombinant mouse Chst5 consists 629 amino acids and predicts a molecular mass of 70.1 kDa.
• Predicted N Terminal: Glu
• Endotoxin: < 1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CHST5 / GlcNAc6ST-3 recombinant protein

A DNA sequence encoding the mouse Chst5 (NP_064334.1) (Ser27-Ser395) was expressed with the Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 9910284
• NCBI GeneID: 56773
• NCBI Accession #: NP_064334.1
• NCBI GenBank Nucleotide #: NM_019950.2
• UniProt Accession #: Q9QUP4
• Molecular Weight: 44,537 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 5
• NCBI Official Synonym Full Names: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
• NCBI Official Symbol: Chst5
• NCBI Official Synonym Symbols: GST-4; Gn6st-3; AI173964; I-GlcNAc-6-ST
• NCBI Protein Information: carbohydrate sulfotransferase 5
• UniProt Protein Name: Carbohydrate sulfotransferase 5
• UniProt Gene Name: Chst5
• UniProt Synonym Gene Names: Gst4; GST4; I-GlcNAc6ST; Intestinal GlcNAc-6-sulfotransferase; mIGn6ST; GlcNAc6ST-3; Gn6st-3

Uniprot Description

CHST6: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N- acetyllactosamine structures. Defects in CHST6 are the cause of macular corneal dystrophy (MCD). MCD is an autosomal recessive disease characterized by corneal opacities. Onset occurs in the first decade, usually between ages 5 and 9. The disorder is progressive. Minute, gray, punctate opacities develop. Corneal sensitivity is usually reduced. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. There are different types of MCD: MCD type I, in which there is a virtual absence of sulfated keratan sulfate (KS) in the serum and cornea, as determined by KS-specific antibodies; and MCD type II, in which the normal sulfated KS-antibody response is present in cornea and serum. MCD type I patients usually have a homozygous missense mutation, while MCD type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 8|8 E1

Cellular Component: integral to membrane

Molecular Function: N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; sulfur metabolic process