Typical Testing Data/Standard Curve (for reference only)
Neurotrophin-3 ELISA Kit | Ntf3 elisa kit
Dog Canine Neurotrophin-3 PicoKine ELISA Kit
Gene Names
Ntf3; Nt3; HDNF; NGF-2; Ntf-3; AI316846; AI835689
Reactivity
CanineThere is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
Applications
ELISA
Synonyms
Neurotrophin-3; Dog Canine Neurotrophin-3 PicoKine ELISA Kit; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor; Ntf3; Ntf-3; Ntf3 elisa kit
Reactivity
Canine
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
Specificity
Natural and recombinant Dog Dog Canine Neurotrophin-3
Sequence Length
258
Applicable Applications for Ntf3 elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Ntf3 elisa kit
Principle of the Assay: Dog Dog Canine Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Dog Canine Neurotrophin-3 has been precoated onto 96-well plates. Standards(Expression system for standard: sf21; Immunogen sequence: Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Dog Canine Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Dog Dog Canine Neurotrophin-3 amount of sample captured in plate.
Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to Dog Caninea chromosome 12p and Dog Caninea chromosome 6.
Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to Dog Caninea chromosome 12p and Dog Caninea chromosome 6.
Product Categories/Family for Ntf3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29588 MW
NCBI Official Full Name
neurotrophin-3 isoform a
NCBI Official Synonym Full Names
neurotrophin 3
NCBI Official Symbol
Ntf3
NCBI Official Synonym Symbols
Nt3; HDNF; NGF-2; Ntf-3; AI316846; AI835689
NCBI Protein Information
neurotrophin-3
UniProt Protein Name
Neurotrophin-3
Protein Family
UniProt Gene Name
Ntf3
UniProt Synonym Gene Names
Ntf-3; NT-3; NGF-2
NCBI Description
This gene encodes a member of the neurotrophins that have a wide variety of functions in both neural and non-neural tissues. The encoded preproprotein undergoes proteolytic processing to generate a noncovalently linked homodimeric mature protein that can bind to the transmembrane receptor tyrosine kinases to initiate a series of signaling events. Mice lacking the encoded protein exhibit severe defects in the peripheral nervous system including a complete lack of spinal proprioceptive afferents and their peripheral sense organs. [provided by RefSeq, Sep 2016]
Uniprot Description
Seems to promote the survival of visceral and proprioceptive sensory neurons.
Research Articles on Ntf3
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Product Notes
The Canine There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine. Ntf3 ntf3 (Catalog #AAA1751050) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751050 ELISA Kit recognizes Canine There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine. Ntf3. AAA Biotech's Neurotrophin-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the Ntf3 ntf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurotrophin-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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