Mouse Neurotrophin 4 ELISA Kit | NT4 elisa kit

Mouse Neurotrophin 4 ELISA Kit

Cat. Num. AAA736113

• Gene Names: NTF4; NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
• Reactivity: Mouse
• Synonyms: Neurotrophin 4; Mouse Neurotrophin 4 ELISA Kit; NT4 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This NT4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse NT4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NT4 elisa kit

Principle of the Assay: NT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NT4 antibody and an NT4-HRP conjugate. The assay sample and buffer are incubated together with NT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT4 concentration since NT4 from samples and NT4-HRP conjugate compete for the anti-NT4 antibody binding site. Since the number of sites is limited, as more sites are occupied by NT4 from the sample, fewer sites are left to bind NT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NT4 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 5453808
• NCBI GeneID: 4909
• NCBI Accession #: NP_006170.1
• NCBI GenBank Nucleotide #: NM_006179.4
• Molecular Weight: 22,427 Da
• NCBI Official Full Name: neurotrophin-4 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 4
• NCBI Official Symbol: NTF4
• NCBI Official Synonym Symbols: NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
• NCBI Protein Information: neurotrophin-4; neurotrophin-5; neutrophic factor 4; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 5 (neurotrophin 4/5)
• UniProt Protein Name: Neurotrophin-4
• UniProt Gene Name: NTF4
• UniProt Synonym Gene Names: NTF5; NT-4; NT-5
• UniProt Entry Name: NTF4_HUMAN

NCBI Description

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-4/5: Target-derived survival factor for peripheral sensory sympathetic neurons. Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O). A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Belongs to the NGF-beta family.

Protein type: Secreted, signal peptide; Cytokine; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: endoplasmic reticulum lumen; cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: neurotrophin p75 receptor binding; growth factor activity

Biological Process: mechanoreceptor differentiation; epidermis development; long-term memory; cell-cell signaling; adult locomotory behavior; regulation of neuron differentiation; sensory organ boundary specification; ganglion mother cell fate determination; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis

Disease: Glaucoma 1, Open Angle, O