Typical Testing Data/Standard Curve (for reference only)
Canine Neurotrophin 4 ELISA Kit | NT4 elisa kit
Canine Neurotrophin 4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NT4 antibody and an NT4-HRP conjugate. The assay sample and buffer are incubated together with NT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT4 concentration since NT4 from samples and NT4-HRP conjugate compete for the anti-NT4 antibody binding site. Since the number of sites is limited, as more sites are occupied by NT4 from the sample, fewer sites are left to bind NT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]
Uniprot Description
NT-4/5: Target-derived survival factor for peripheral sensory sympathetic neurons. Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O). A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Belongs to the NGF-beta family.
Protein type: Secreted, signal peptide; Cytokine; Secreted; Cell development/differentiation
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: endoplasmic reticulum lumen; cytoplasmic membrane-bound vesicle; extracellular region
Molecular Function: neurotrophin p75 receptor binding; growth factor activity
Biological Process: epidermis development; mechanoreceptor differentiation; long-term memory; cell-cell signaling; adult locomotory behavior; regulation of neuron differentiation; sensory organ boundary specification; ganglion mother cell fate determination; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis
Disease: Glaucoma 1, Open Angle, O
Research Articles on NT4
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Product Notes
The Canine NT4 ntf4 (Catalog #AAA749491) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA749491 ELISA Kit recognizes Canine NT4. It is sometimes possible for the material contained within the vial of "Neurotrophin 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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