Rat Neurotrophin-4 ELISA Kit | NT-4 elisa kit

Rat Neurotrophin-4 ELISA Kit

Cat. Num. AAA723822

• Gene Names: NTF4; NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
• Reactivity: Rat
• Synonyms: Neurotrophin-4; Rat Neurotrophin-4 ELISA Kit; NT-4 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/ml
• Intended Uses: This NT-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NT-4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NT-4 elisa kit

Principle of the Assay: NT-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NT-4. Standards or samples are then added to the microtiter plate wells and NT-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NT-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for NT-4 are added to each well to "sandwich" the NT-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NT-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT-4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NT-4 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 5453808
• NCBI GeneID: 4909
• NCBI Accession #: NP_006170.1
• NCBI GenBank Nucleotide #: NM_006179.4
• Molecular Weight: 22,427 Da
• NCBI Official Full Name: neurotrophin-4 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 4
• NCBI Official Symbol: NTF4
• NCBI Official Synonym Symbols: NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
• NCBI Protein Information: neurotrophin-4; neurotrophin-5; neutrophic factor 4; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 5 (neurotrophin 4/5)
• UniProt Protein Name: Neurotrophin-4
• Protein Family: Neurotrophin
• UniProt Gene Name: NTF4
• UniProt Synonym Gene Names: NTF5; NT-4; NT-5
• UniProt Entry Name: NTF4_HUMAN

NCBI Description

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-4/5: Target-derived survival factor for peripheral sensory sympathetic neurons. Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O). A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Belongs to the NGF-beta family.

Protein type: Cytokine; Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: endoplasmic reticulum lumen; cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: neurotrophin p75 receptor binding; growth factor activity

Biological Process: epidermis development; mechanoreceptor differentiation; cell-cell signaling; long-term memory; regulation of neuron differentiation; adult locomotory behavior; sensory organ boundary specification; ganglion mother cell fate determination; negative regulation of neuron apoptosis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Glaucoma 1, Open Angle, O