Rabbit anti-Human, Mouse Caspase-9 Polyclonal Antibody | anti-CASP9 antibody

Caspase-9 Polyclonal Antibody

Cat. Num. AAA3012368

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Caspase-9; Polyclonal Antibody; Caspase-9 Polyclonal Antibody; CASP-9; APAF-3; ICE-LAP6; CASP9; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; Caspase 9 apoptosis related cysteine peptidase; Caspase 9 Dominant Negative; Caspase 9c; Caspase-9 subunit p10; ICE LAP6; ICE like apoptotic protease 6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1; regulatory subunit 56; RNCASP9; anti-CASP9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase-9 polyclonal antibody detects endogenous levels of Caspase-9 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 416

• Applicable Applications for anti-CASP9 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:2,000
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: C2C12)

Related Product Information for anti-CASP9 antibody

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

NCBI and Uniprot Product Information

• NCBI GI #: 14790124
• NCBI GeneID: 842
• NCBI Accession #: NP_001220.2
• NCBI GenBank Nucleotide #: NP_001220.2
• UniProt Accession #: P55211; Q8C3Q9
• Molecular Weight: 46/39/37/35kDa
• NCBI Official Full Name: caspase-9 isoform alpha
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Apoptosis; EC 3.4.22.62; Protease

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: mitochondrion; nucleus; cytosol; apoptosome

Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging