Human Neurotrophin-3 ELISA Kit | NT-3 elisa kit

Human Neurotrophin-3 ELISA Kit

Cat. Num. AAA762605

• Gene Names: NTF3; NT3; HDNF; NGF2; NT-3; NGF-2
• Reactivity: Human
• Synonyms: Neurotrophin-3; Human Neurotrophin-3 ELISA Kit; NT-3 (Neurotrophin-3)/NTF3/3-nucleotidase; NT-3 elisa kit

• Reactivity: Human
• Sequence Length: 270

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NT-3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human NT- 3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NT-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NT-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NT-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 156630995
• NCBI GeneID: 4908
• NCBI Accession #: NP_001096124.1
• NCBI GenBank Nucleotide #: NM_001102654.1
• Molecular Weight: 30,800 Da
• NCBI Official Full Name: neurotrophin-3 isoform 1 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 3
• NCBI Official Symbol: NTF3
• NCBI Official Synonym Symbols: NT3; HDNF; NGF2; NT-3; NGF-2
• NCBI Protein Information: neurotrophin-3
• UniProt Protein Name: Neurotrophin-3
• Protein Family: Neurotrophin
• UniProt Gene Name: NTF3
• UniProt Synonym Gene Names: NT-3; NGF-2
• UniProt Entry Name: NTF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: chemoattractant activity; growth factor activity; nerve growth factor binding; neurotrophin p75 receptor binding; protein binding; receptor binding

Biological Process: activation of MAPK activity; activation of protein kinase B; axon guidance; brain development; cell-cell signaling; enteric nervous system development; epidermis development; glial cell fate determination; induction of positive chemotaxis; mechanoreceptor differentiation; myelination; negative regulation of neuron apoptosis; negative regulation of peptidyl-tyrosine phosphorylation; nerve development; nervous system development; neurite morphogenesis; neuromuscular synaptic transmission; peripheral nervous system development; positive chemotaxis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of glial cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of receptor internalization; positive regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of neuron differentiation; regulation of synaptic transmission; signal transduction; smooth muscle cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway