Sheep MGST1 ELISA Kit | MGST1 elisa kit

Sheep MGST1 (Microsomal Glutathione S Transferase 1) ELISA Kit

Cat. Num. AAA2504932

• Gene Names: MGST1; MGST; GST12; MGST-I
• Reactivity: Sheep
• Synonyms: MGST1; Sheep MGST1 (Microsomal Glutathione S Transferase 1) ELISA Kit; MGST1 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep MGST1. No significant cross-reactivity or interference between Sheep MGST1 and analogues was observed.
• Sequence Length: 87

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MGST1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep MGST1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MGST1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MGST1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MGST1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MGST1. You can calculate the concentration of MGST1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 390125204
• NCBI GeneID: 4257
• NCBI Accession #: NP_001254527.1
• NCBI GenBank Nucleotide #: NM_001267598.1
• UniProt Accession #: P10620; A8K533; G5EA53
• Molecular Weight: 9,984 Da
• NCBI Official Full Name: microsomal glutathione S-transferase 1 isoform c
• NCBI Official Synonym Full Names: microsomal glutathione S-transferase 1
• NCBI Official Symbol: MGST1
• NCBI Official Synonym Symbols: MGST; GST12; MGST-I
• NCBI Protein Information: microsomal glutathione S-transferase 1; glutathione S-transferase 12; microsomal GST-1; microsomal GST-I
• UniProt Protein Name: Microsomal glutathione S-transferase 1
• Protein Family: Microsomal glutathione S-transferase
• UniProt Gene Name: MGST1
• UniProt Synonym Gene Names: GST12; MGST; Microsomal GST-1
• UniProt Entry Name: MGST1_HUMAN

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

MGST1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. Belongs to the MAPEG family.

Protein type: Membrane protein, integral; Mitochondrial; Other Amino Acids Metabolism - glutathione; Transferase; Membrane protein, multi-pass; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 12p12.3-p12.1

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; apical part of cell; endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: protein binding; protein homodimerization activity; glutathione transferase activity; glutathione binding; glutathione peroxidase activity

Biological Process: response to drug; Leydig cell differentiation; glutathione metabolic process; xenobiotic metabolic process; response to lipopolysaccharide; response to organic nitrogen