Immunohistochemistry (IHC)
(MBS9604095 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse REV1 Polyclonal Antibody | anti-REV1 antibody
REV1 Antibody
Gene Names
REV1; REV1L; AIBP80
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
REV1; Polyclonal Antibody; REV1 Antibody; AIBP 80; AIBP80; Alpha integrin binding protein 80; Alpha integrin-binding protein 80; DNA repair protein REV 1; DNA repair protein REV1; FLJ21523; MGC163283; MGC26225; REV 1 (yeast homolog) like; REV 1; REV 1 homolog; REV 1 like; REV 1 protein; REV 1L; REV1 (yeast homolog) like; REV1 homolog (S. cerevisiae); REV1 homolog; REV1 like (yeast); REV1 like; Rev1 like terminal deoxycytidyl transferase; REV1 protein; Rev1-like terminal deoxycytidyl transferase; REV1_HUMAN; REV1L; anti-REV1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
REV1 antibody detects endogenous levels of total REV1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1250
Applicable Applications for anti-REV1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (90%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Immunohistochemistry (IHC)
(MBS9604095 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604095 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of REV1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of REV1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-REV1 antibody
Description: Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Subunit Structure: Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. May bind ITGA3. Interacts with FAAP20/C1orf86.
Similarity: The C-terminal domain is necessary for protein interactions. Belongs to the DNA polymerase type-Y family.
Subunit Structure: Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. May bind ITGA3. Interacts with FAAP20/C1orf86.
Similarity: The C-terminal domain is necessary for protein interactions. Belongs to the DNA polymerase type-Y family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 138 kDa
Predicted: 139 kDa
Predicted: 139 kDa
NCBI Official Full Name
DNA repair protein REV1 isoform 2
NCBI Official Synonym Full Names
REV1, DNA directed polymerase
NCBI Official Symbol
REV1
NCBI Official Synonym Symbols
REV1L; AIBP80
NCBI Protein Information
DNA repair protein REV1
UniProt Protein Name
DNA repair protein REV1
Protein Family
UniProt Gene Name
REV1
UniProt Synonym Gene Names
REV1L; AIBP80
NCBI Description
This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
Uniprot Description
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Research Articles on REV1
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Product Notes
The REV1 rev1 (Catalog #AAA9604095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The REV1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's REV1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the REV1 rev1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REV1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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