LRRTM4 recombinant protein

LRRTM4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120804

• Purity: >97% as determined by SDS-PAGE
• Synonyms: LRRTM4; LRRTM4 Protein; Human; Recombinant (His Tag); Human LRRTM4 Protein (His Tag); leucine rich repeat transmembrane neuronal 4; LRRTM4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys424

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 31
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human LRRTM4 extracellular domain (NP_001128217.1)(Met 1-Lys 424) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LRRTM4 recombinant protein

Background: Leucine-rich repeat transmembrane neuronal protein 4, also known as LRRTM4, is a single-pass type I membrane protein that belongs to the LRRTM family. LRRTM4 is expressed in the limb mesenchyme, neural tube, caudal mesoderm, and in three distinct regions of the head. LRRTM4 may play a role in the development and maintenance of the vertebrate nervous system. Leucine-rich repeat-containing proteins are involved in protein-protein interactions and they regulate numerous cellular events during nervous system development and disease. Human and mouse LRRTMs are highly conserved, and orthologous genes exist in other vertebrates but not in invertebrates. LRRTM mRNAs are predominantly expressed in the nervous system and that each LRRTM possesses a specific, partially nonoverlapping expression pattern. The structure and expression profile of LRRTM mRNAs suggest that they may have a role in the development and maintenance of the vertebrate nervous system. All LRRTMs, except LRRTM4, are located in the introns of different alpha-catenin genes, suggesting coevolution of these two gene families.

References

Laura©n,J. et al., 2003, Genomics 81 (4):411-21. Clark HF. et al.,2003, Genome Res. 13: 2265-70. Haines,BP. et al., 2007,Gene Expr Patterns 7 (1-2): 23-9.

NCBI and Uniprot Product Information

• NCBI GI #: 197927168
• NCBI GeneID: 80059
• NCBI Accession #: NP_001128217.1
• NCBI GenBank Nucleotide #: NM_001134745.1
• UniProt Accession #: Q86VH4; Q4FZ98; Q6UXJ7
• Molecular Weight: 67,217 Da
• NCBI Official Full Name: leucine-rich repeat transmembrane neuronal protein 4 isoform a
• NCBI Official Synonym Full Names: leucine rich repeat transmembrane neuronal 4
• NCBI Official Symbol: LRRTM4
• NCBI Protein Information: leucine-rich repeat transmembrane neuronal protein 4; leucine-rich repeat transmembrane neuronal 4 protein
• UniProt Protein Name: Leucine-rich repeat transmembrane neuronal protein 4
• Protein Family: Leucine-rich repeat transmembrane neuronal protein
• UniProt Gene Name: LRRTM4
• UniProt Entry Name: LRRT4_HUMAN

Uniprot Description

LRRTM4: May play a role in the development and maintenance of the vertebrate nervous system. Belongs to the LRRTM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: postsynaptic membrane; cell junction

Molecular Function: heparan sulfate proteoglycan binding