NSUN2 recombinant protein

Recombinant NSUN2 protein

Cat. Num. AAA389226

• Gene Names: NSUN2; MISU; MRT5; SAKI; TRM4
• Synonyms: NSUN2; Recombinant NSUN2 protein; NOP2/Sun RNA Methyltransferase Family Member 2; Myc-Induced SUN-Domain-Containing Protein; MISU; SAKI; NSUN2 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant NSUN2 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant NSUN2 protein 10% SDS-PAGE Coomassie staining MW: 88.1 kDa Purity: >80%)

Related Product Information for NSUN2 recombinant protein

Short Description: Recombinant NSUN2 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_060225.4) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 88.1 kDa.

Background: NSUN2 (NOP2/Sun Domain Family, Member 2), also called as TRM4 (TRNA Methyltransferase 4 Homolog), is a member of NSUN family. It's a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. NSUN2 also methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of introncontaining tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. It may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

Product Categories/Family for NSUN2 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 39995082
• NCBI GeneID: 54888
• NCBI Accession #: NP_060225.4
• NCBI GenBank Nucleotide #: NM_017755.6
• UniProt Accession #: Q08J23; Q9BVN4; Q9H858; Q9NXD9; A8K529; B2RNR4; B3KP09; B4DQW2; G3V1R4
• Molecular Weight: 82,393 Da
• NCBI Official Full Name: tRNA (cytosine(34)-C(5))-methyltransferase isoform 1
• NCBI Official Synonym Full Names: NOP2/Sun RNA methyltransferase family member 2
• NCBI Official Symbol: NSUN2
• NCBI Official Synonym Symbols: MISU; MRT5; SAKI; TRM4
• NCBI Protein Information: tRNA (cytosine(34)-C(5))-methyltransferase
• UniProt Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase
• Protein Family: tRNA
• UniProt Gene Name: NSUN2
• UniProt Synonym Gene Names: SAKI; TRM4; Misu; hTrm4
• UniProt Entry Name: NSUN2_HUMAN

NCBI Description

This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NSUN2: an RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. Interacts with NPM1 and NCL during interphase. Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA. Defects in NSUN2 are the cause of mental retardation autosomal recessive type 5 (MRT5), a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. Two human isoforms produced by alternative splicing.

Protein type: EC 2.1.1.203; Methyltransferase; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 5p15.31

Cellular Component: cytoplasm; nucleolus; spindle; nucleus

Molecular Function: tRNA (cytosine-5-)-methyltransferase activity; tRNA binding

Biological Process: mitosis; cell division; tRNA methylation

Disease: Mental Retardation, Autosomal Recessive 5