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Western Blot (WB)
(Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: 293)
Rabbit anti-Human, Mouse NSUN4 Polyclonal Antibody | anti-NSUN4 antibody
NSUN4 Antibody
Gene Names
NSUN4; SHTAP
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
NSUN4; Polyclonal Antibody; NSUN4 Antibody; 5 methylcytosine rRNA methyltransferase NSUN4 antibody; EC=2.1.1.- antibody; NOL1/NOP2/Sun domain family member 4 antibody; NOL1/NOP2/Sun domain family; member 4 antibody; NOP2/Sun domain family; Nsun4 antibody; NSUN4_HUMAN antibody; Putative methyltransferase NSUN4 antibody; RP4-603I14.2 antibody; Shtap antibody; Sperm head and tail associated protein antibody; anti-NSUN4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NSUN4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: 293)
Western Blot (WB)
(Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: 293)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-NSUN4 antibody
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Product Categories/Family for anti-NSUN4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,374 Da
NCBI Official Full Name
5-methylcytosine rRNA methyltransferase NSUN4 isoform a
NCBI Official Synonym Full Names
NOP2/Sun domain family, member 4
NCBI Official Symbol
NSUN4
NCBI Official Synonym Symbols
SHTAP
NCBI Protein Information
5-methylcytosine rRNA methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; putative methyltransferase NSUN4; sperm head and tail associated protein
UniProt Protein Name
5-methylcytosine rRNA methyltransferase NSUN4
Protein Family
UniProt Gene Name
NSUN4
UniProt Entry Name
NSUN4_HUMAN
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Product Notes
The NSUN4 nsun4 (Catalog #AAA9435129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NSUN4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NSUN4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the NSUN4 nsun4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSUN4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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