NRXN3 recombinant protein

NRXN3 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120995

• Gene Names: NRXN3; C14orf60
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NRXN3; NRXN3 Protein; Human; Recombinant (hFc Tag); Human Neurexin-3-beta/NRXN3 Protein (Fc Tag); C14orf60 Protein; neurexin 3; NRXN3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr357

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 36
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human NRXN3 isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NRXN3 recombinant protein

Background: Neurexin-3-beta, also known as Neurexin III-beta and NRXN3, is a single-pass type I membrane protein that belongs to the neurexin family. It contains one laminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes (NRXN1, NRXN2, NRXN3) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain EGF-like sequences and laminin G domains and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. NRXN3 has been linked to a genetic predisposition towards some conditions such as alcohol or drug addiction, or obesity.

References

Occhi G. et al., 2002, Biochem. Biophys. Res. Commun. 298:151-5. Rowen L, et al., 2002, Genomics. 79 (4): 587-97. Olsen J.V. et al., 2006, Cell 127:635-48. Hishimoto A, et al., 2007, Human Molecular Genetics.16 (23): 2880-91. Oppermann F.S. et al., 2009, Mol. Cell. Proteomics 8:1751-64.

NCBI and Uniprot Product Information

• NCBI GI #: 41350305
• NCBI GeneID: 9369
• NCBI Accession #: NP_620426.2
• NCBI GenBank Nucleotide #: NM_138970.4
• UniProt Accession #: Q9HDB5; Q6NUR0; Q8IUD8; A5PKW8; A8MPU5; B3KPM7; Q9Y4C0
• Molecular Weight: 180,599 Da
• NCBI Official Full Name: neurexin 3 isoform 2
• NCBI Official Synonym Full Names: neurexin 3
• NCBI Official Symbol: NRXN3
• NCBI Official Synonym Symbols: C14orf60
• NCBI Protein Information: neurexin 3
• UniProt Protein Name: Neurexin-3-beta
• Protein Family: Neurexin
• UniProt Gene Name: NRXN3
• UniProt Synonym Gene Names: KIAA0743; NRXN3-CTF
• UniProt Entry Name: NRX3B_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants and protein isoforms for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. Genetic variation at this locus has been associated with a range of behavioral phenotypes, including alcohol dependence and autism spectrum disorder. [provided by RefSeq, Dec 2012]

Uniprot Description

NRXN3B: Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May play a role in angiogenesis. {ECO:0000250}. Belongs to the neurexin family. {ECO:0000305}. The cytoplasmic C-terminal region binds to CASK. Binds to neuroligins NLGN1, NLGN2 and NLGN3. Weakly interacts with CBLN1 and CBLN2. Very weak binding, if any, with CBLN4. {ECO:0000250}. 7 isoforms of the human protein are produced by alternative promoter

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: integral to plasma membrane; integral to membrane

Molecular Function: metal ion binding; calcium channel regulator activity; receptor activity; cell adhesion molecule binding

Biological Process: synaptic transmission; axon guidance; positive regulation of synaptogenesis; synaptogenesis; neurotransmitter secretion; adult behavior; neuron adhesion; learning; angiogenesis; social behavior