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Rabbit Procollagen I N-Terminal Propeptide (PINP) Polyclonal Antibody | anti-PINP antibody
Rabbit anti-human Procollagen I N-Terminal Propeptide (PINP) Polyclonal antibody
Reactivity
Human, Mouse, Rat, Dog, Cow, Horse, Sheep.
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Rabbit Polyclonal IgG, the antibody was affinity-purified by Protein A from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Procollagen I N-Terminal Propeptide (PINP); Polyclonal Antibody; Rabbit anti-human Procollagen I N-Terminal Propeptide (PINP) Polyclonal antibody; P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; anti-PINP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Cow, Horse, Sheep.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Rabbit Polyclonal IgG, the antibody was affinity-purified by Protein A from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin 300 and 50% Glycerol.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-PINP antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry - Frozen/Paraffin (IHC-F/P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Not yet tested in other applications.
Not yet tested in other applications.
Application Notes
WB=1:500-2000
EIA=1:500-1000
IHC-P=1:400-800
IHC-F=1:400-800
ICC=1:100-500
IF=1:100-500
Optimal working dilutions must be determined by the end user.
EIA=1:500-1000
IHC-P=1:400-800
IHC-F=1:400-800
ICC=1:100-500
IF=1:100-500
Optimal working dilutions must be determined by the end user.
Immunogen
KLH conjugated synthetic peptide derived from human Procollagen type I N terminal propeptide (P02452).
Preparation and Storage
Storage: Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**
Stability: Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
Stability: Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
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Related Product Information for anti-PINP antibody
The PINP molecule is similar to PIIINP consisting of three distinct structural domains: Col 1 is on the aminoterminal side of the molecule, while Col 2 and Col 3 are situated on the middle of the helically structured molecule (Kühn et al. 1982). The PINP molecule has a molecular mass of 35 000 and is cleared by scavenger receptors in liver endothelial cells (Melkko et al. 1994). PINP often occurs in circulation in two forms of different molecular sizes. One is identical to the trimeric authentic antigen (intact PINP) whereas the other consists of smaller forms of PINP, resembling a single domain of the proalpha1(I) chain of PINP and is probably a degradation product of type I procollagen or I pN-collagen. Thus, an assay of intact PINP rather than total PINP appears to be more sensitive in detecting changes in the rate of type I collagen synthesis (Melkko et al. 1996, Risteli & Risteli 1999).
Similar Products
Product Notes
The PINP (Catalog #AAA286271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Procollagen I N-Terminal Propeptide (PINP) Polyclonal antibody reacts with Human, Mouse, Rat, Dog, Cow, Horse, Sheep. and may cross-react with other species as described in the data sheet. AAA Biotech's Procollagen I N-Terminal Propeptide (PINP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry - Frozen/Paraffin (IHC-F/P), Immunocytochemistry (ICC), Immunofluorescence (IF) Not yet tested in other applications. WB=1:500-2000 EIA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 Optimal working dilutions must be determined by the end user. Researchers should empirically determine the suitability of the PINP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Procollagen I N-Terminal Propeptide (PINP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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