Neuregulin 1 (NRG1) Recombinant Protein | NRG1 recombinant protein

Recombinant Neuregulin 1 (NRG1)

Cat. Num. AAA2009710

• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Neuregulin 1 (NRG1); Recombinant Neuregulin 1 (NRG1); NRG1 recombinant protein

• Host: E.coli
• Purity/Purification: > 95%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin 300.
• Concentration: Original Concentration: 200ug/mL (varies by lot)

• Applicable Applications for NRG1 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Organism Species: Rattus norvegicus (Rat)
• Source: Prokaryotic expression
• Residues: Thr264~Gly393
• Tags: N-terminal His Tag
• Subcellular Location: Membrane, Secreted
• Traits: Freeze-dried powder
• Predicted isoelectric point: 9.3
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1.Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage: ; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months. ; ; Stability Test: ; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dateunder appropriate storage condition.

SDS-Page

Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 402794669
• NCBI GeneID: 112400
• NCBI Accession #: NP_001258052.1
• NCBI GenBank Nucleotide #: NM_001271123.1
• UniProt Accession #: P43322; P43323; P43324; P43325; P43326; P43327; P43328
• Molecular Weight: Predicted Molecular Mass: 15.7kDa; Accurate Molecular Mass: 20/18kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: pro-neuregulin-1, membrane-bound isoform isoform 6
• NCBI Official Synonym Full Names: neuregulin 1
• NCBI Official Symbol: Nrg1
• NCBI Protein Information: pro-neuregulin-1, membrane-bound isoform; pro-NRG1; neuregulin 1 type III beta 3
• UniProt Protein Name: Pro-neuregulin-1, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: Nrg1
• UniProt Synonym Gene Names: Ndf; Pro-NRG1; ARIA; HRG
• UniProt Entry Name: NRG1_RAT

NCBI Description

ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]

Uniprot Description

NRG1: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. Belongs to the neuregulin family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Cell development/differentiation; Membrane protein, integral; Cytokine; Motility/polarity/chemotaxis

Cellular Component: extracellular space; integral to plasma membrane; axon; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; synapse; neuromuscular junction

Molecular Function: ErbB-2 class receptor binding; ErbB-3 class receptor binding; growth factor activity; transcription cofactor activity; protein tyrosine kinase activator activity; receptor binding

Biological Process: myelination; regulation of protein heterodimerization activity; muscle development; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; wound healing; cellular protein complex disassembly; heart development; ventricular cardiac muscle cell differentiation; cell morphogenesis; locomotory behavior; glucose transport; synaptogenesis; glial cell differentiation; regulation of blood pressure; regulation of cell differentiation; organ development; cell differentiation; positive regulation of cardiac muscle cell proliferation; nervous system development; cell migration; neurotransmitter receptor metabolic process; MAPKKK cascade; neuron fate commitment; positive regulation of cell growth; peripheral nervous system development; cell proliferation; positive regulation of protein kinase B signaling cascade; glial cell fate commitment; positive regulation of protein kinase activity; negative regulation of secretion; positive regulation of Ras protein signal transduction; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway