Neuregulin 1 (NRG1) Recombinant Protein | NRG1 recombinant protein

Recombinant Neuregulin 1 (NRG1)

Cat. Num. AAA2009319

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Neuregulin 1 (NRG1); Recombinant Neuregulin 1 (NRG1); NRG1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-GKKKDRGS RGKPGPAEGD PSPALPPRLK EMKSQESAAG SKLVLRCETS SEYSSLRFKW FKNGNELNRK NKPENIKIQK KPGKSELRIN KASLADSGEY MCKVISKLGN DSASANITIV ESNEFITGMP ASTETAYVSS ESPIRISVST EGANTSSSTS TSTTGTSHLI KCAEKEKTFC VNGGECFTVK DLSNPSRYLC KCPNEFTGDR CQNYVMASFY KAEELYQKRV LTITGICIAL LVVGIMCVV
• Sequence Length: 241

• Applicable Applications for NRG1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly13~Val259 (Accession # P43322) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 402794669
• NCBI GeneID: 112400
• NCBI Accession #: NP_001258052.1
• NCBI GenBank Nucleotide #: NM_001271123.1
• UniProt Accession #: P43322; P43323; P43324; P43325; P43326; P43327; P43328
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: pro-neuregulin-1, membrane-bound isoform isoform 6
• NCBI Official Synonym Full Names: neuregulin 1
• NCBI Official Symbol: Nrg1
• NCBI Protein Information: pro-neuregulin-1, membrane-bound isoform; pro-NRG1; neuregulin 1 type III beta 3
• UniProt Protein Name: Pro-neuregulin-1, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: Nrg1
• UniProt Synonym Gene Names: Ndf; Pro-NRG1; ARIA; HRG
• UniProt Entry Name: NRG1_RAT

NCBI Description

ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]

Uniprot Description

NRG1: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. Belongs to the neuregulin family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Motility/polarity/chemotaxis; Cell development/differentiation; Membrane protein, integral; Ligand, receptor tyrosine kinase

Cellular Component: extracellular space; axon; integral to plasma membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; synapse; neuromuscular junction

Molecular Function: ErbB-2 class receptor binding; ErbB-3 class receptor binding; growth factor activity; transcription cofactor activity; protein tyrosine kinase activator activity; receptor binding

Biological Process: myelination; muscle development; regulation of protein heterodimerization activity; positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); cellular protein complex disassembly; wound healing; heart development; cell morphogenesis; ventricular cardiac muscle cell differentiation; locomotory behavior; glucose transport; synaptogenesis; glial cell differentiation; regulation of blood pressure; regulation of cell differentiation; organ development; cell differentiation; positive regulation of cardiac muscle cell proliferation; nervous system development; cell migration; neurotransmitter receptor metabolic process; MAPKKK cascade; neuron fate commitment; positive regulation of cell growth; peripheral nervous system development; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of protein kinase activity; glial cell fate commitment; negative regulation of secretion; positive regulation of Ras protein signal transduction; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway