Rat Neuregulin 1 (NRG1) RTU ELISA Kit | NRG1 rtu elisa kit

Rat Neuregulin 1 (NRG1) ELISA Kit

Cat. Num. AAA4501472

• Reactivity: Rat
• Synonyms: Neuregulin 1 (NRG1); Rat Neuregulin 1 (NRG1) ELISA Kit; ARIA; GGF; HGL; HRG; HRGA; NDF; SMDF; Acetylcholine receptor-inducing activity; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor; NRG1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 241

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.225ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRG1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NRG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NRG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 402794669
• NCBI GeneID: 112400
• NCBI Accession #: NP_001258052.1
• NCBI GenBank Nucleotide #: NM_001271123.1
• UniProt Accession #: P43322; P43323; P43324; P43325; P43326; P43327; P43328
• Molecular Weight: 26,213 Da
• NCBI Official Full Name: pro-neuregulin-1, membrane-bound isoform isoform 6
• NCBI Official Synonym Full Names: neuregulin 1
• NCBI Official Symbol: Nrg1
• NCBI Protein Information: pro-neuregulin-1, membrane-bound isoform
• UniProt Protein Name: Pro-neuregulin-1, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: Nrg1
• UniProt Synonym Gene Names: Ndf; Pro-NRG1; ARIA; HRG

NCBI Description

ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]

Uniprot Description

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Binds to ERBB4 and ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling (). Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1, and ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (PubMed:17250808).