Human natriuretic peptide precursor A ELISA Kit | NPPA elisa kit

Human atrial natriuretic peptide, ANP ELISA Kit

Cat. Num. AAA701832

• Gene Names: NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• Reactivity: Human
• Synonyms: natriuretic peptide precursor A; Human atrial natriuretic peptide; ANP ELISA Kit; Human atrial natriuretic peptide (ANP) ELISA kit; ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptides A (NPPA) ; NPPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NPPA. No significant cross-reactivity or interference between Human NPPA and analogues was observed.
• Sequence Length: 151

• Samples: Serum, cell culture supernates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPPA elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 23510319
• NCBI GeneID: 4878
• NCBI Accession #: NP_006163.1
• NCBI GenBank Nucleotide #: NM_006172.3
• UniProt Accession #: P01160; Q13766; Q5JZE1
• Molecular Weight: 16,708 Da
• NCBI Official Full Name: natriuretic peptides A preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: NPPA
• NCBI Official Synonym Symbols: ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• NCBI Protein Information: natriuretic peptides A; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A
• UniProt Protein Name: Natriuretic peptides A
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANP; PND; CDP; ANF; ANP
• UniProt Entry Name: ANF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.1 PublicationManual assertion based on experiment in:Ref.15