Porcine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit | NT-ProBNP elisa kit

Porcine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

Cat. Num. AAA268024

• Gene Names: NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• Reactivity: Porcine
• Synonyms: N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP); Porcine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit; NT-ProBNP elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 151

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.05 ng/ml
• Sensitivity: Up to 0.05 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NT-ProBNP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine NT-ProBNP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 23510319
• NCBI GeneID: 4878
• NCBI Accession #: NP_006163.1
• NCBI GenBank Nucleotide #: NM_006172.3
• Molecular Weight: 16,708 Da
• NCBI Official Full Name: natriuretic peptides A preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: NPPA
• NCBI Official Synonym Symbols: ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• NCBI Protein Information: natriuretic peptides A; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A
• UniProt Protein Name: Natriuretic peptides A
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANP; PND; CDP; ANF; ANP
• UniProt Entry Name: ANF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; extracellular region; nucleus

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor binding

Biological Process: cardiac muscle hypertrophy; cGMP biosynthetic process; transcription initiation from RNA polymerase II promoter; neuropeptide signaling pathway; regulation of blood pressure; negative regulation of systemic arterial blood pressure; receptor guanylyl cyclase signaling pathway; response to hypoxia; gene expression; positive regulation of heart rate; negative regulation of cell growth; female pregnancy; regulation of blood vessel size; response to insulin stimulus

Disease: Atrial Standstill 2; Atrial Fibrillation, Familial, 6