Mouse anti-Human LST1 Antibody | anti-LST1 antibody

Anti-LST1 Purified

Cat. Num. AAA1800870

• Gene Names: LST1; B144; LST-1; D6S49E
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: LST1; Anti-LST1 Purified; leukocyte specific transcript 1; LST-1; B144; anti-LST1 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: LST1/02
• Specificity: The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 73

• Applicable Applications for anti-LST1 antibody: Immunoprecipitation, Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen: KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line.Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-06)Lane 2-3: immunostaining with anti-human LST1 (LST1/02), two independent batches.Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.)

Related Product Information for anti-LST1 antibody

LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A - LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

NCBI and Uniprot Product Information

• NCBI GI #: 262118183
• NCBI GeneID: 7940
• NCBI Accession #: NP_001160010.1
• NCBI GenBank Nucleotide #: NP_001160010.1
• UniProt Accession #: O00453
• NCBI Official Full Name: leukocyte-specific transcript 1 protein isoform 6
• NCBI Official Synonym Full Names: leukocyte specific transcript 1
• NCBI Official Symbol: LST1
• NCBI Official Synonym Symbols: B144; LST-1; D6S49E
• NCBI Protein Information: leukocyte-specific transcript 1 protein
• UniProt Protein Name: Leukocyte-specific transcript 1 protein
• Protein Family: Leukocyte-specific transcript 1 protein
• UniProt Gene Name: LST1
• UniProt Synonym Gene Names: B144
• UniProt Entry Name: LST1_HUMAN

NCBI Description

The protein encoded by this gene is a membrane protein that can inhibit the proliferation of lymphocytes. Expression of this gene is enhanced by lipopolysaccharide, interferon-gamma, and bacteria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

LST1: Possible role in modulating immune responses. Induces morphological changes including production of filopodia and microspikes when overexpressed in a variety of cell types and may be involved in dendritic cell maturation. Isoform 1 and isoform 2 have an inhibitory effect on lymphocyte proliferation. Belongs to the LST1 family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; integral to membrane

Biological Process: regulation of cell shape; anatomical structure morphogenesis; cell morphogenesis; dendrite development; immune response; negative regulation of lymphocyte proliferation