NMNAT2 / NMNAT-2 recombinant protein

Recombinant Human NMNAT2 / NMNAT-2 Protein (His tag)

Cat. Num. AAA2545791

• Gene Names: NMNAT2; PNAT2; C1orf15
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: NMNAT2 / NMNAT-2; Recombinant Human NMNAT2 / NMNAT-2 Protein (His tag); NMNAT2; C1orf15; KIAA0479; NMNAT2 / NMNAT-2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 5mM DTT, 10% glycerol, pH 8.0
• Sequence Length: 307

• Application Notes: The recombinant human NMNAT2 consists of 317 amino acids and has a calculated molecular mass of 35.8 kDa. The recombinant protein migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NMNAT2 / NMNAT-2 recombinant protein

Background: NMNAT2, also known as NMNAT-2, belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family. NMNAT is a central enzyme in NAD+ biosynthesis, transferring the adenylyl moiety of ATP to nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) resulting in the formation of NAD+ or NaAD+ and the release of pyrophosphate. NMNAT2 is predominantly expressed in human pancreas, insulinoma as well as in the brain, especially in the cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Immunofluorescence microscopy localized endogenous NMNAT2 to the Golgi apparatus in human cell line. Endogenous NMNAT2 seem to be a labile axon survival factor, because specific depletion of NMNAT2 is sufficient to induce Wallerian-like degeneration of uninjured axons which endogenous NMNAT1 and NMNAT3 cannot prevent. Thus endogenous NMNAT2 represents an exciting new therapeutic target for axonal disorders.

Description: A DNA sequence encoding the human NMNAT2 (Q9BZQ4-1) (Met1-Gly307) was fused with a polyhistide tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 24307989
• NCBI GeneID: 23057
• NCBI Accession #: NP_055854.1
• NCBI GenBank Nucleotide #: NM_015039.3
• UniProt Accession #: Q9BZQ4; O75067; Q5T1Q3; Q8WU99; Q96QW1
• Molecular Weight: 34,015 Da
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2 isoform 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 2
• NCBI Official Symbol: NMNAT2
• NCBI Official Synonym Symbols: PNAT2; C1orf15
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
• UniProt Gene Name: NMNAT2
• UniProt Synonym Gene Names: C1orf15; KIAA0479; NMN/NaMN adenylyltransferase 2; NMN adenylyltransferase 2; NaMN adenylyltransferase 2

NCBI Description

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NMNAT2: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Belongs to the eukaryotic NMN adenylyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.7.7.1; EC 2.7.7.18; Transferase

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: Golgi apparatus; Golgi membrane

Molecular Function: nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity

Biological Process: NAD biosynthetic process; NAD metabolic process