NMNAT2 recombinant protein

NMNAT2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120809

• Gene Names: NMNAT2; PNAT2; C1orf15
• Purity: >80% as determined by SDS-PAGE
• Synonyms: NMNAT2; NMNAT2 Protein; Human; Recombinant (His Tag); Human NMNAT2/NMNAT-2 Protein (His Tag); C1orf15 Protein; PNAT2 Protein; nicotinamide nucleotide adenylyltransferase 2; NMNAT2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 5mM DTT, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly307

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human NMNAT2 (Q9BZQ4-1)(Met1-Gly307) was fused with a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NMNAT2 recombinant protein

Background: NMNAT2, also known as NMNAT-2, belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family. NMNAT is a central enzyme in NAD+ biosynthesis, transferring the adenylyl moiety of ATP to nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) resulting in the formation of NAD+ or NaAD+ and the release of pyrophosphate. NMNAT2 is predominantly expressed in the human pancreas, insulinoma as well as in the brain, especially in the cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe, and putamen. Immunofluorescence microscopy localized endogenous NMNAT2 to the Golgi apparatus in the human cell line. Endogenous NMNAT2 seems to be a labile axon survival factor because specific depletion of NMNAT2 is sufficient to induce Wallerian-like degeneration of uninjured axons which endogenous NMNAT1 and NMNAT3 cannot prevent. Thus endogenous NMNAT2 represents an exciting new therapeutic target for axonal disorders.

References

Ljungberg MC. et al., 2012, Hum Mol Genet. 21 (2): 251-67. Seki N. et al., 1998, DNA Res. 4 (5): 345-9. Raffaelli N. et al., 2002, Biochem Biophys Res Commun. 297 (4): 835-40. Sood R. et al., 2001, Genomics. 73 (2): 211-22.

NCBI and Uniprot Product Information

• NCBI GI #: 24307989
• NCBI GeneID: 23057
• NCBI Accession #: NP_055854.1
• NCBI GenBank Nucleotide #: NM_015039.3
• UniProt Accession #: Q9BZQ4; O75067; Q5T1Q3; Q8WU99; Q96QW1
• Molecular Weight: 34,015 Da
• NCBI Official Full Name: nicotinamide mononucleotide adenylyltransferase 2 isoform 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 2
• NCBI Official Symbol: NMNAT2
• NCBI Official Synonym Symbols: PNAT2; C1orf15
• NCBI Protein Information: nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NaMN adenylyltransferase 2; nicotinate-nucleotide adenylyltransferase 2; pyridine nucleotide adenylyltransferase 2
• UniProt Protein Name: Nicotinamide mononucleotide adenylyltransferase 2
• UniProt Gene Name: NMNAT2
• UniProt Synonym Gene Names: C1orf15; KIAA0479; NMN adenylyltransferase 2; NaMN adenylyltransferase 2
• UniProt Entry Name: NMNA2_HUMAN

NCBI Description

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NMNAT2: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Belongs to the eukaryotic NMN adenylyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.7.7.1

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi membrane; Golgi apparatus; late endosome; synapse; trans-Golgi network

Molecular Function: nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process