Rabbit DHFR Polyclonal Antibody | anti-DHFR antibody

DHFR (Dihydrofolate Reductase, DYR, DHFRP1) (HRP)

Cat. Num. AAA6414026

• Gene Names: DHFR; DYR; DHFRP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: DHFR; Polyclonal Antibody; DHFR (Dihydrofolate Reductase; DYR; DHFRP1) (HRP); anti-DHFR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes DHFR. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-DHFR antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human DHFR.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DHFR antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using DHFR antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using DHFR antibody at dilution of 1:100 (200x lens).)

Product Categories/Family for anti-DHFR antibody

Antibodies; Enzymes; Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 118992
• NCBI GeneID: 1719
• UniProt Accession #: P00374; Q14130; Q6IRW8; B4DDD2
• Molecular Weight: 187
• NCBI Official Full Name: Dihydrofolate reductase
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: DHFR
• NCBI Official Synonym Symbols: DYR; DHFRP1
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Viral dihydrofolate reductase
• UniProt Gene Name: DHFR
• UniProt Entry Name: DYR_HUMAN

NCBI Description

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.3; Cofactor and Vitamin Metabolism - folate biosynthesis

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: mRNA binding; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; vitamin metabolic process; one-carbon compound metabolic process; nitric oxide metabolic process; G1/S-specific transcription in mitotic cell cycle; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; response to methotrexate; regulation of nitric-oxide synthase activity; folic acid metabolic process; mitotic cell cycle; glycine biosynthetic process; water-soluble vitamin metabolic process; G1/S transition of mitotic cell cycle

Disease: Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency