NMNAT1 / NMNAT recombinant protein

Recombinant Human NMNAT1 / NMNAT Protein (His tag)

Cat. Num. AAA2545778

• Gene Names: NMNAT1; LCA9; NMNAT; PNAT1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: NMNAT1 / NMNAT; Recombinant Human NMNAT1 / NMNAT Protein (His tag); NMNAT1; NMNAT; NMNAT1 / NMNAT recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4
• Sequence Length: 279

• Application Notes: The secreted recombinant human NMNAT1 consists of 289 amino acids and predicts a molecular mass of 33.3 KDa. The apparent molecular mass of the protein is approximately 34 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NMNAT1 / NMNAT recombinant protein

Background: NMNAT, also known as NMNAT1, is a member of the Nicotinamide-nucleotide adenylyltransferases. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. NMNAT appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca (2+) signaling pathways. NMNAT enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.

Description: A DNA sequence encoding the human NMNAT1 (Q9HAN9) (Met 1-Thr279) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 663071195
• NCBI GeneID: 64802
• NCBI Accession #: NP_001284707.1
• NCBI GenBank Nucleotide #: NM_001297778.1
• UniProt Accession #: Q9HAN9; Q8TAE9; Q9H247; Q9H6B6; B1AN63
• Molecular Weight: 31,932 Da
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 1
• NCBI Official Symbol: NMNAT1
• NCBI Official Synonym Symbols: LCA9; NMNAT; PNAT1
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Gene Name: NMNAT1
• UniProt Synonym Gene Names: NMNAT; NMN/NaMN adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1

NCBI Description

This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]

Uniprot Description

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+ (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD+ and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+ (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults ().