Bovine NF-kappa-B inhibitor zeta ELISA Kit | NFKBIZ elisa kit

Bovine NF-kappa-B inhibitor zeta (NFKBIZ) ELISA Kit

Cat. Num. AAA285888

• Gene Names: NFKBIZ; MAIL
• Reactivity: Bovine
• Synonyms: NF-kappa-B inhibitor zeta; Bovine NF-kappa-B inhibitor zeta (NFKBIZ) ELISA Kit; FLJ30225; FLJ34463; IKBZ; INAP; MAIL; Ikappa B-zeta variant 3|IkappaB-zeta|molecule possessing ankyrin repeats induced by lipopolysaccharide; NFKBIZ elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine NFKBIZ. No significant cross-reactivity or interference between Bovine NFKBIZ and analogues was observed.
• Sequence Length: 618

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for NFKBIZ elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NFKBIZ in samples. An antibody specific for NFKBIZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFKBIZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKBIZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKBIZ bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 41386701
• NCBI GeneID: 282713
• NCBI Accession #: NP_777151.1
• NCBI GenBank Nucleotide #: NM_174726.1
• UniProt Accession #: Q9BE45; A7Z059
• Molecular Weight: 78,239 Da
• NCBI Official Full Name: NF-kappa-B inhibitor zeta
• NCBI Official Synonym Full Names: NFKB inhibitor zeta
• NCBI Official Symbol: NFKBIZ
• NCBI Official Synonym Symbols: MAIL
• NCBI Protein Information: NF-kappa-B inhibitor zeta
• UniProt Protein Name: NF-kappa-B inhibitor zeta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIZ
• UniProt Synonym Gene Names: MAIL; IkB-zeta; IkappaBzeta; MAIL

Uniprot Description

Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis ().