Western Blot (WB)
(The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human DNAL1 Monoclonal Antibody | anti-DNAL1 antibody
DNAL1 antibody
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF)
(ICC/IF analysis of DNAL1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of DNAL1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of DNAL1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of DNAL1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]
Uniprot Description
Part of the multisubunit axonemal ATPase complexes that generate the force for cilia motility and govern beat frequency (). Component of the outer arm dynein (ODA). May be involved in a mechanosensory feedback mechanism controlling ODA activity based on external conformational cues by tethering the outer arm dynein heavy chain (DNAH5) to the microtubule within the axoneme (). Important for ciliary function in the airways and for the function of the cilia that produce the nodal flow essential for the determination of the left-right asymmetry (PubMed:21496787).
Research Articles on DNAL1
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Product Notes
The DNAL1 dnal1 (Catalog #AAA200321) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNAL1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the DNAL1 dnal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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