Typical Testing Data/Standard Curve (for reference only)
Rat Nuclear factor erythroid 2-related factor 2 ELISA Kit | Nfe2l2 elisa kit
Rat Nuclear factor erythroid 2-related factor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-E2-related factor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-E2-related factor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-E2-related factor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NF-E2-related factor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
a basic leucine zipper transcription factor; involved in activating expression of genes with an antioxidant response element (ARE) [RGD, Feb 2006]
Uniprot Description
NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.
Protein type: DNA-binding; Transcription factor
Cellular Component: centrosome; chromatin; cytoplasm; cytosol; nucleoplasm; nucleus; plasma membrane
Molecular Function: DNA binding; protein binding; protein domain specific binding; sequence-specific DNA binding; transcription factor activity
Biological Process: cell redox homeostasis; cellular response to glucose starvation; inflammatory response; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of blood vessel endothelial cell migration; positive regulation of glucose import; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of embryonic development; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; unfolded protein response
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Product Notes
The Rat Nfe2l2 nfe2l2 (Catalog #AAA2881763) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881763 ELISA Kit recognizes Rat Nfe2l2. It is sometimes possible for the material contained within the vial of "Nuclear factor erythroid 2-related factor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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