Mouse Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit | NFE2L2 elisa kit

Mouse Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit

Cat. Num. AAA2024760

• Gene Names: NFE2L2; NRF2
• Reactivity: Mouse
• Synonyms: Nuclear Factor; Erythroid Derived 2 Like Protein 2 (NFE2L2); Mouse Nuclear Factor; Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit; NRF2; HEBP1; NF-E2-Related Factor 2; Nuclear factor; erythroid derived 2; like 2; NFE2L2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2).; No significant cross-reactivity or interference between Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) and analogues was observed.
• Sequence Length: 605

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFE2L2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NFE2L2 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 25453452
• NCBI GeneID: 4780
• Molecular Weight: 65,356 Da
• NCBI Official Full Name: Nuclear factor erythroid 2-related factor 2
• NCBI Official Synonym Full Names: nuclear factor, erythroid 2-like 2
• NCBI Official Symbol: NFE2L2
• NCBI Official Synonym Symbols: NRF2
• NCBI Protein Information: nuclear factor erythroid 2-related factor 2
• UniProt Protein Name: Nuclear factor erythroid 2-related factor 2
• Protein Family: Nuclear factor erythroid 2-related factor
• UniProt Gene Name: NFE2L2
• UniProt Synonym Gene Names: NRF2; NF-E2-related factor 2; NFE2-related factor 2
• UniProt Entry Name: NF2L2_HUMAN

NCBI Description

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; chromatin; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter