Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit | IRF9 elisa kit

Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit

Cat. Num. AAA2706499

• Gene Names: IRF9; p48; IRF-9; ISGF3; ISGF3G
• Reactivity: Human
• Synonyms: Interferon Regulatory Factor 9 (IRF9); Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit; ISGF3G; ISGF3; p48; Interferon-Stimulated Transcription Factor 3; Gamma 48kDa; IFN-alpha-responsive transcription factor subunit; Interferon-stimulated gene factor 3 gamma; IRF9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Regulatory Factor 9 (IRF9).; No significant cross-reactivity or interference between Interferon Regulatory Factor 9 (IRF9) and analogues was observed.
• Sequence Length: 393

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.048ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Regulatory Factor 9 (IRF9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Regulatory Factor 9 (IRF9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRF9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 9 (IRF9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 9 (IRF9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 9 (IRF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Regulatory Factor 9 (IRF9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 25282407
• NCBI GeneID: 10379
• NCBI Accession #: NP_006075.3
• NCBI GenBank Nucleotide #: NM_006084.4
• UniProt Accession #: Q00978; D3DS61
• Molecular Weight: 43,696 Da
• NCBI Official Full Name: interferon regulatory factor 9
• NCBI Official Synonym Full Names: interferon regulatory factor 9
• NCBI Official Symbol: IRF9
• NCBI Official Synonym Symbols: p48; IRF-9; ISGF3; ISGF3G
• NCBI Protein Information: interferon regulatory factor 9
• UniProt Protein Name: Interferon regulatory factor 9
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF9
• UniProt Synonym Gene Names: ISGF3G; IRF-9; ISGF-3 gamma

Uniprot Description

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.