Nell2 recombinant protein

NELL2 Recombinant Protein

Cat. Num. AAA153296

• Gene Names: Nell2; mel91; R75516; A330108N19Rik
• Applications: ELISA, Western Blot
• Purity: ~95%
• Synonyms: NELL2; NELL2 Recombinant Protein; NELL2 Recombinant Protein: mel91; R75516; A330108N19Rik; NEL-like 2; Nell2 recombinant protein

• Purity/Purification: ~95%
• Form/Format: Liquid
• Sequence: aa 22 - 238
• Sequence Length: 816

• Applicable Applications for Nell2 recombinant protein: ELISA (EIA), Western Blot (WB), MS
• Application Notes: This recombinant protein can be used for WB, ELISA, MS and neutralization assays. For research use only.
• Species: Mouse
• Source: E Coli
• Buffer: 100mM sodium phosphate, 10mM Tris, 500mM NaCl, 25 mM imidazole, 2mM MgCl2, 10% gycerol
• Fusion Tag: Fusion Partner: C-terminal His-tag
• Preparation and Storage: Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

SDS-PAGE

(SDS-PAGE analysis of recombinant NELL2 fragment on Coomassie Blue-stained 12% SDS-PAGE gel.)

Related Product Information for Nell2 recombinant protein

NELL2 is a neuron-specific thrombospondin-1-like extracellular protein containing six epidermal growth factor-like domains (1). It is highly expressed in the hippocampus in vivo (2) and promotes survival of rat primary cultured neurons through activation of JNK and suppression of ERK (3). As ERK is required for the induction of hippocampal long term potentiation (LTP) and JNK appears to be inhibitory on LTP (4,5), it had been suggested that NELL2 may play a role in synaptic plasticity. More recently, NELL2 has been shown to promote differentiation of motor and sensory neurons and stimulates mitogenesis of dorsal root ganglia in chickens (6).

NCBI and Uniprot Product Information

• NCBI GI #: 2494290
• NCBI GeneID: 54003
• UniProt Accession #: Q61220; Q80UM5
• Molecular Weight: 28 kDa (Calculated)
• NCBI Official Full Name: Protein kinase C-binding protein NELL2
• NCBI Official Synonym Full Names: NEL-like 2
• NCBI Official Symbol: Nell2
• NCBI Official Synonym Symbols: mel91; R75516; A330108N19Rik
• NCBI Protein Information: protein kinase C-binding protein NELL2; NEL-like protein 2; nel-like 2 homolog
• UniProt Protein Name: Protein kinase C-binding protein NELL2
• Protein Family: Protein kinase C-binding protein
• UniProt Gene Name: Nell2
• UniProt Synonym Gene Names: Mel91
• UniProt Entry Name: NELL2_MOUSE

Uniprot Description

NELL2: is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; membrane; dendrite; cytoplasm; extracellular region; perikaryon

Molecular Function: heparin binding; identical protein binding; protein kinase C binding; calcium ion binding

Biological Process: regulation of growth; positive regulation of hormone secretion; development of secondary female sexual characteristics