Negative elongation factor E (RDBP) Recombinant Protein | RDBP recombinant protein

Recombinant Human Negative elongation factor E (RDBP)

Cat. Num. AAA950285

• Gene Names: NELFE; RD; RDP; RDBP; D6S45; NELF-E
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Negative elongation factor E (RDBP); Recombinant Human Negative elongation factor E (RDBP); RDBP recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-380, Full length protein
• Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF
• Sequence Length: 380

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for RDBP recombinant protein

This protein is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

NCBI and Uniprot Product Information

• NCBI GI #: 14670268
• NCBI GeneID: 7936
• NCBI Accession #: NP_002895.3
• NCBI GenBank Nucleotide #: NM_002904.5
• UniProt Accession #: P18615; Q5JP74; Q5JP75; Q96F56; Q9NPK2; A2BE08; B4DUN1; B4DYX9
• Molecular Weight: 40,216 Da
• NCBI Official Full Name: negative elongation factor E
• NCBI Official Synonym Full Names: negative elongation factor complex member E
• NCBI Official Symbol: NELFE
• NCBI Official Synonym Symbols: RD; RDP; RDBP; D6S45; NELF-E
• NCBI Protein Information: negative elongation factor E
• UniProt Protein Name: Negative elongation factor E
• UniProt Gene Name: NELFE
• UniProt Synonym Gene Names: RD; RDBP; NELF-E

NCBI Description

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.