NEDD8 recombinant protein

NEDD8 (human), (recombinant) (Biotin)

Cat. Num. AAA566712

• Gene Names: NEDD8; NEDD-8
• Synonyms: NEDD8; NEDD8 (human); (recombinant) (Biotin); NEDD8 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized. Contains PBS

• Reconstitution: Reconstitute with sterile distilled water
• Preparation and Storage: Stable for at least 12 months after receipt when stored at -20 degree C. Avoid freeze/thaw cycles; Shipping: Shipped on Blue Ice

Related Product Information for NEDD8 recombinant protein

Biotinylated NEDD8 is a useful reagent for studying NEDDylation in vitro, where it represents a readily detectable alternative to radio-iodinated NEDD8. Biotinylated NEDD8 is activated by NEDD8 E1, is a competent substrate for the NEDD8 E2 conjugating enzyme UbcH12 and can be conjugated to target proteins in the presence of a suitable NEDD8 E3 ligase. The biotinyl moiety also provides a potential route to affinity purification of conjugated substrates (or thiol ester-linked enzymes) on avidin/streptavidin matrices. NEDD8 contains 9 lysine residues, any/many of which are likely to be biotinylated in this product. These modification sites have not been mapped. Biotinylated NEDD8 is prepared from recombinant human His-tagged NEDD8.

Product Categories/Family for NEDD8 recombinant protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 5453760
• NCBI GeneID: 4738
• NCBI Accession #: NP_006147.1
• NCBI GenBank Nucleotide #: NM_006156.2
• UniProt Accession #: Q15843; Q3SXN8; Q6LES6
• Molecular Weight: 9,072 Da
• NCBI Official Full Name: NEDD8
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 8
• NCBI Official Symbol: NEDD8
• NCBI Official Synonym Symbols: NEDD-8
• NCBI Protein Information: NEDD8; neddylin; ubiquitin-like protein Nedd8
• UniProt Protein Name: NEDD8
• Protein Family: NEDD8
• UniProt Gene Name: NEDD8
• UniProt Synonym Gene Names: NEDD-8
• UniProt Entry Name: NEDD8_HUMAN

Uniprot Description

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; protein localization; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance