NEDD8 recombinant protein

Recombinant Human NEDD8

Cat. Num. AAA2569224

• Gene Names: NEDD8; NEDD-8
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: NEDD8; Recombinant Human NEDD8; Neural precursor cell expressed developmentally down-regulated protein 8; Neddylin; Ubiquitin-like protein Nedd8; NEDD8 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of 20mM Tris-HCl, 250mM NaCl, pH8.0.
• Sequence: Met1-Gly76

• Species: Human
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Neural precursor cell expressed developmentally down-regulated protein 8 is produced by our E Coli expression system and the target gene encoding Met1-Gly76 is expressed.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for NEDD8 recombinant protein

Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

Product Categories/Family for NEDD8 recombinant protein

Cell biology

NCBI and Uniprot Product Information

• NCBI GI #: 5453760
• NCBI GeneID: 4738
• NCBI Accession #: NP_006147.1
• NCBI GenBank Nucleotide #: NM_006156.2
• UniProt Accession #: Q15843; Q3SXN8; Q6LES6
• Molecular Weight: 9,072 Da
• NCBI Official Full Name: NEDD8
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 8
• NCBI Official Symbol: NEDD8
• NCBI Official Synonym Symbols: NEDD-8
• NCBI Protein Information: NEDD8; neddylin; ubiquitin-like protein Nedd8
• UniProt Protein Name: NEDD8
• Protein Family: NEDD8
• UniProt Gene Name: NEDD8
• UniProt Synonym Gene Names: NEDD-8
• UniProt Entry Name: NEDD8_HUMAN

Uniprot Description

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; protein localization; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance