Goat ADAM12 Polyclonal Antibody | anti-ADAM12 antibody

Goat Polyclonal to Human ADAM12

Cat. Num. AAA242207

• Gene Names: ADAM12; MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• Reactivity: Human; Predicted Reactivity: Gorilla (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ADAM12; Polyclonal Antibody; Goat Polyclonal to Human ADAM12; Anti-ADAM12 Antibody (N-Terminus) IHC-plus; MCMPMltna; MCMP; MLTN; Meltrin-alpha; MLTNA; Human ADAM12; anti-ADAM12 antibody

• Host: Goat
• Reactivity: Human; Predicted Reactivity: Gorilla (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ADAM12. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 909

• Applicable Applications for anti-ADAM12 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide AARPLPVSPARALC from the N-terminus of human ADAM12 (NP_003465.3; NP_067673.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: ADAM12 antibody was raised against synthetic peptide AARPLPVSPARALC from the N-terminus of human ADAM12 (NP_003465.3; NP_067673.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ADAM12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody staining (0.1 ug/ml) of human heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Related Product Information for anti-ADAM12 antibody

ADAM12 is a Metallopeptidase M12B type protease.

Product Categories/Family for anti-ADAM12 antibody

Family: Protease; Subfamily: Metallopeptidase M12B

NCBI and Uniprot Product Information

• NCBI GI #: 73747885
• NCBI GeneID: 8038
• NCBI Accession #: NP_003465.3
• NCBI GenBank Nucleotide #: NM_003474.5
• UniProt Accession #: O43184; O60470; Q5JRP0; Q5JRP1; Q6P9E3; Q6UWB0
• Molecular Weight: 80,263 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 12 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 12
• NCBI Official Symbol: ADAM12
• NCBI Official Synonym Symbols: MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 12
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM12
• UniProt Synonym Gene Names: MLTN; ADAM 12
• UniProt Entry Name: ADA12_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

Uniprot Description

ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.

Protein type: Membrane protein, integral; Protease; Motility/polarity/chemotaxis; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; mitochondrion; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; proteolysis; cell adhesion; myoblast fusion