Rabbit NEDD8 Polyclonal Antibody | anti-NEDD8 antibody

NEDD8 Antibody

Cat. Num. AAA9410074

• Gene Names: NEDD8; NEDD-8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: NEDD8; Polyclonal Antibody; NEDD8 Antibody; FLJ43224; MGC104393; MGC125896; MGC125897; anti-NEDD8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NEDD8 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 81

• Applicable Applications for anti-NEDD8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human NEDD8.
• Target Name: NEDD8
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NEDD8 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using NEDD8 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NEDD8 antibody

Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family (1,2). The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination. NEDD8 is first synthesized in a precursor form with a carboxy-terminal extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein (3,4). Mature NEDD8 is then covalently linked to target proteins via the carboxy-terminal glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively (5). An E3 ligase protein, Roc1/Rbx1, is also required for neddylation of the cullin proteins (6). Protein de-neddylation is catalyzed by a number of enzymes in the cell, including a "ubiquitin-specific" protease USP21, the NEDP1/DEN1 hydrolase and the COP9/signalosome (CSN) (7,8,9). In contrast to the ubiquitin pathway, the NEDD8 modification system acts on only a few substrates and does not appear to target proteins for degradation. Neddylation of cullin proteins activates the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex by promoting complex formation and enhancing the recruitment of the E2-ubiquitin intermediate (10). While NEDD8 modification of VHL is not required for ubiquitination of HIF1-a, it is required for fibronectin matrix assembly (11). Mdm2-dependent neddylation of p53 inhibits its transcriptional activity (12).

Product Categories/Family for anti-NEDD8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5453760
• NCBI GeneID: 4738
• NCBI Accession #: NP_006147.1
• NCBI GenBank Nucleotide #: NM_006156.2
• UniProt Accession #: Q15843; Q3SXN8; Q6LES6
• Molecular Weight: 9,072 Da
• NCBI Official Full Name: NEDD8
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 8
• NCBI Official Symbol: NEDD8
• NCBI Official Synonym Symbols: NEDD-8
• NCBI Protein Information: NEDD8
• UniProt Protein Name: NEDD8
• Protein Family: NEDD8
• UniProt Gene Name: NEDD8
• UniProt Synonym Gene Names: NEDD-8
• UniProt Entry Name: NEDD8_HUMAN

Uniprot Description

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; protein localization; protein neddylation; anatomical structure morphogenesis; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance