SDS-Page
NEDD8 recombinant protein
NEDD8, 1-76aa, Human, His tag, E Coli
Gene Names
NEDD8; NEDD-8
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
NEDD8; 1-76aa; Human; His tag; E Coli; Neural precursor cell expressed developmentally down-regulated 8; ubiquitin-like protein Nedd8; Neddylin; Neural precursor cell expressed; developmentally down-regulated 8 MGC104393; MGC125896; MGC125897; NED8; 0NEDD 8; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Rub1; ubiquitin like protein Nedd 8; ubiquitin like protein Nedd8.; NEDD8 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG
Sequence Length
81
Applicable Applications for NEDD8 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for NEDD8 recombinant protein
NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E Coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
Product Categories/Family for NEDD8 recombinant protein
References
Wu K., et al. (2002) J Biol Chem. 277 (1): 516-27; Hori T., et al. (1999) Oncogene. 18 (48): 6829-34;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12.8 kDa (113 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
NCBI Protein Information
NEDD8
UniProt Protein Name
NEDD8
Protein Family
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
Uniprot Description
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Research Articles on NEDD8
Similar Products
Product Notes
The NEDD8 nedd8 (Catalog #AAA203213) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's NEDD8 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NEDD8 nedd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG. It is sometimes possible for the material contained within the vial of "NEDD8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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