Carnitine-O-Octanoyltransferase Recombinant Protein | CORT recombinant protein

Recombinant Carnitine-O-Octanoyltransferase (CORT)

Cat. Num. AAA2033329

• Gene Names: CROT; COT
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Carnitine-O-Octanoyltransferase; Recombinant Carnitine-O-Octanoyltransferase (CORT); COT; CROT; Peroxisomal Carnitine O-Octanoyltransferase; CORT recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: K NKMLHPDTFI QLALQLAYYR LHGHPGCCYE TAMTRHFYHG RTETMRSCTV EAVRWCQSMQ DPSVNLRERQ QKMLQAFAKH NKMMKDCSAG KGFDRHLLGL LLIAKEEGLP VPELFTDPLF SKSGGGGNFV LSTSLVGYLR VQGVVVPMVH NGYGFFYHIRDDRFVVACSA WKSCPETDAE KLVQLTFCAF HDMIQLMNST HL
• Sequence Length: 612

• Applicable Applications for CORT recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 26.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Lys410~Leu612
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119597360
• NCBI GeneID: 54677
• Molecular Weight: 73,383 Da
• NCBI Official Full Name: carnitine O-octanoyltransferase
• NCBI Official Synonym Full Names: carnitine O-octanoyltransferase
• NCBI Official Symbol: CROT
• NCBI Official Synonym Symbols: COT
• NCBI Protein Information: peroxisomal carnitine O-octanoyltransferase
• UniProt Protein Name: Peroxisomal carnitine O-octanoyltransferase
• Protein Family: Cortistatin
• UniProt Gene Name: CROT
• UniProt Synonym Gene Names: COT; COT
• UniProt Entry Name: OCTC_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Uniprot Description

CROT: Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester. Belongs to the carnitine/choline acetyltransferase family.

Protein type: Transferase; EC 2.3.1.137

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: intracellular membrane-bound organelle; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: carnitine O-octanoyltransferase activity; receptor binding

Biological Process: carnitine metabolic process; cellular lipid metabolic process; coenzyme A metabolic process; fatty acid beta-oxidation; fatty acid beta-oxidation using acyl-CoA oxidase; fatty acid metabolic process; fatty acid transport; generation of precursor metabolites and energy; medium-chain fatty acid metabolic process; response to drug; response to organic nitrogen