Human Nectin-4 ELISA Kit | NECTIN4 elisa kit

Human Nectin-4 PicoKine ELISA Kit

Cat. Num. AAA178569

• Gene Names: NECTIN4; LNIR; PRR4; EDSS1; PVRL4; nectin-4
• Reactivity: Human
• Synonyms: Nectin-4; Human Nectin-4 PicoKine ELISA Kit; EDSS1; Nectin 4; PRR4; pvrl4; LNIR; Q96NY8; nectin cell adhesion molecule 4; NECTIN4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Nectin-4
• Sequence Length: 510

• Samples: Cell culture supernates, serum, plasma(heparin, EDTA), cell lysates, tissue homogenates and urine.
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human Nectin-4 PicoKine ELISA Kit standard curve )

Related Product Information for NECTIN4 elisa kit

Principle of the Assay: Human Nectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nectin-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: G32-S349) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Nectin-4 amount of sample captured in plate.

Background/Introduction: Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 222136611
• NCBI GeneID: 81607
• NCBI Accession #: NP_112178.2
• NCBI GenBank Nucleotide #: NM_030916.2
• UniProt Accession #: Q96NY8; Q96K15; B4DQW3
• Molecular Weight: 24,341 Da
• NCBI Official Full Name: nectin-4
• NCBI Official Synonym Full Names: nectin cell adhesion molecule 4
• NCBI Official Symbol: NECTIN4
• NCBI Official Synonym Symbols: LNIR; PRR4; EDSS1; PVRL4; nectin-4
• NCBI Protein Information: nectin-4
• UniProt Protein Name: Nectin-4
• Protein Family: Nectin
• UniProt Gene Name: NECTIN4
• UniProt Entry Name: NECT4_HUMAN

NCBI Description

This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]

Uniprot Description

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.