Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuroendocrine convertase 1 (PCSK1) Standard Curve.)
Rat Neuroendocrine Convertase 1 (PCSK1) ELISA Kit | NEC1 elisa kit
Rat Neuroendocrine Convertase 1 (PCSK1) ELISA Kit
Gene Names
Pcsk1; BDP; PC1; PC3
Reactivity
Rat
Synonyms
Neuroendocrine Convertase 1 (PCSK1); Rat Neuroendocrine Convertase 1 (PCSK1) ELISA Kit; PCSK1; BMIQ12; PC1; PC3; SPC3; neuroendocrine convertase 1; prohormone convertase 1; prohormone convertase 3; proprotein convertase 1; NEC1 elisa kit
Reactivity
Rat
Specificity
Rat Neuroendocrine convertase 1 (PCSK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PCSK1. No significant cross-reactivity or interference between Rat PCSK1 and analogues was observed.
Sequence Length
752
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuroendocrine convertase 1 (PCSK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuroendocrine convertase 1 (PCSK1) Standard Curve.)
Related Product Information for NEC1 elisa kit
Description: This Rat Neuroendocrine convertase 1 (PCSK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK1 in samples. An antibody specific for PCSK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCSK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proprotein Convertase Subtilisin/Kexin Type 1 is aprotein belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known.
Background: Proprotein Convertase Subtilisin/Kexin Type 1 is aprotein belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neuroendocrine convertase 1
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 1
NCBI Official Symbol
Pcsk1
NCBI Official Synonym Symbols
BDP; PC1; PC3
NCBI Protein Information
neuroendocrine convertase 1
UniProt Protein Name
Neuroendocrine convertase 1
Protein Family
UniProt Gene Name
Pcsk1
UniProt Synonym Gene Names
Bdp; Nec-1; Nec1; NEC 1; PC1
NCBI Description
plays a role in proteolytic processing of peptide hormone and other protein precursors [RGD, Feb 2006]
Uniprot Description
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
Research Articles on NEC1
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Product Notes
The Rat NEC1 pcsk1 (Catalog #AAA9712142) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712142 ELISA Kit recognizes Rat NEC1. It is sometimes possible for the material contained within the vial of "Neuroendocrine Convertase 1 (PCSK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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