Rabbit anti-Human FAKD3 Polyclonal Antibody | anti-FAKD3 antibody

Rabbit FAKD3 Antibody

Cat. Num. AAA4117

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
• Synonyms: FAKD3; Polyclonal Antibody; Rabbit FAKD3 Antibody; anti-FAKD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FAKD3 Antibody detects endogenous levels of total FAKD3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-FAKD3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500; IHC: 1:50-1:200,; ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human FAKD3, corresponding to a region within N-terminal amino acids.
• Important: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using FAKD3 antibody.The lane on the left was treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9604743 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunofluorescence (IF)

(Staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 40068497
• NCBI GeneID: 79072
• NCBI Accession #: NP_076996.2
• NCBI GenBank Nucleotide #: NM_024091.3
• UniProt Accession #: Q14CZ7; Q9BVD3
• Molecular Weight: 75 KD
• NCBI Official Full Name: FAST kinase domain-containing protein 3
• NCBI Official Synonym Full Names: FAST kinase domains 3
• NCBI Official Symbol: FASTKD3
• NCBI Protein Information: FAST kinase domain-containing protein 3
• UniProt Protein Name: FAST kinase domain-containing protein 3
• UniProt Gene Name: FASTKD3
• UniProt Entry Name: FAKD3_HUMAN

NCBI Description

This gene encodes a member of a small family of Fas-activated serine/threonine kinase domain (FASTKD) containing proteins that share an amino terminal mitochondrial targeting domain and multiple carboxy terminal FAST domains as well as a putative RNA-binding RAP domain. The members of this family are ubiquitously expressed and are generally most abundant in mitochondria-enriched tissues such as heart, skeletal muscle and brown-adipose tissue. Some members of this protein family may play a role in apoptosis. The protein encoded by this gene interacts with components of the mitochondrial respiratory and translation networks. A pseudogene of this gene is also present on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

FASTKD3: Belongs to the FAST kinase family.

Chromosomal Location of Human Ortholog: 5p15.31

Cellular Component: mitochondrion

Molecular Function: protein kinase activity

Biological Process: cellular respiration; protein amino acid phosphorylation