Rat SMARCA4 Monoclonal Antibody | anti-SMARCA4 antibody

SMARCA4 antibody (mAb)(Clone 5B7)

Cat. Num. AAA388983

• Gene Names: SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
• Reactivity: Human, Monkey, Mouse
• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Protein G Chromatography
• Synonyms: SMARCA4; Monoclonal Antibody; SMARCA4 antibody (mAb)(Clone 5B7); BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; anti-SMARCA4 antibody

• Host: Rat
• Reactivity: Human, Monkey, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5B7
• Purity/Purification: Protein G Chromatography
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-SMARCA4 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications Validated:; ICC/IF: 2-10ug/ml dilution; WB: 0.5-2ug/ml dilution
• Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 213-295 of human SMARCA4.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Immunofluorescence (IF)

(SMARCA4 antibody (mAb) (Clone 5B7) tested by immunofluorescence. Top: HeLa cells stained with SMARCA4 antibody (mAb). Bottom: Hoechst staining.)

Western Blot (WB)

(SMARCA4 antibody (mAb) (Clone 5B7) tested by Western blot. Whole cell extracts were probed with SMARCA4 antibody (mAb). Lane 1: L929 cells. Lane 2: HeLa cells. Lane 3: Cos cells)

Related Product Information for anti-SMARCA4 antibody

Short Description: Our SMARCA4 antibody (mAb)(Clone 5B7) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human, Monkey and Mouse samples.

Background: SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4) is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency.

Product Categories/Family for anti-SMARCA4 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 192807323
• NCBI GeneID: 6597
• NCBI Accession #: NP_001122321; AB_2793708
• NCBI GenBank Nucleotide #: NM_001128849.1
• UniProt Accession #: P51532; O95052; Q9HBD3; B1A8Z4; B1A8Z5; B1A8Z6; B1A8Z7; E9PBR8
• Molecular Weight: Predicted: 185 kDa; ; Observed: 200 kDa
• NCBI Official Full Name: transcription activator BRG1 isoform A
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• NCBI Official Symbol: SMARCA4
• NCBI Official Synonym Symbols: BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
• NCBI Protein Information: transcription activator BRG1; SNF2-beta; SNF2-like 4; protein BRG-1; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; protein brahma homolog 1; BRG1-associated factor 190A; sucrose nonfermenting-like 4; ATP-de
• UniProt Protein Name: Transcription activator BRG1
• Protein Family: Transcription activator
• UniProt Gene Name: SMARCA4
• UniProt Synonym Gene Names: BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
• UniProt Entry Name: SMCA4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]