NCF-2 / NCF2 / P67phox recombinant protein

Recombinant Human NCF-2 / NCF2 / P67phox Protein (His & GST tag)

Cat. Num. AAA2545220

• Gene Names: NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: NCF-2 / NCF2 / P67phox; Recombinant Human NCF-2 / NCF2 / P67phox Protein (His & GST tag); NCF2; NOXA2; P67PHOX; NCF-2 / NCF2 / P67phox recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4
• Sequence Length: 526

• Application Notes: The recombinant human NCF2 /GST chimera consists of 763 amino acids and has a calculated molecular mass of 87.6 kDa. The recombinant protein migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for NCF-2 / NCF2 / P67phox recombinant protein

Background: NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.

Description: A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 12804409
• NCBI GeneID: 4688
• Molecular Weight: 50,337 Da
• NCBI Official Full Name: Neutrophil cytosolic factor 2
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 2
• NCBI Official Symbol: NCF2
• NCBI Official Synonym Symbols: NCF-2; NOXA2; P67PHOX; P67-PHOX
• NCBI Protein Information: neutrophil cytosol factor 2
• UniProt Protein Name: Neutrophil cytosol factor 2
• UniProt Gene Name: NCF2
• UniProt Synonym Gene Names: NOXA2; P67PHOX; NCF-2

NCBI Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: cytoplasm; cytosol; NADPH oxidase complex; nucleolus

Molecular Function: electron carrier activity; protein binding; protein C-terminus binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; cellular defense response; innate immune response; respiratory burst; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii