Typical Testing Data/Standard Curve (for reference only)
Human Transcription factor HES-1 ELISA Kit | HES1 elisa kit
Human Transcription factor HES-1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
Uniprot Description
HES1: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
Protein type: Transcription factor; Cell development/differentiation; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3q28-q29
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: chaperone binding; DNA binding; histone deacetylase binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: adenohypophysis development; artery morphogenesis; auditory receptor cell fate determination; cell adhesion; cell maturation; cell migration; endocrine pancreas development; hindbrain morphogenesis; lateral inhibition; liver development; lung development; midbrain development; midbrain-hindbrain boundary morphogenesis; negative regulation of auditory receptor cell differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; neuron morphogenesis during differentiation; Notch signaling pathway; oculomotor nerve development; positive regulation of astrocyte differentiation; positive regulation of BMP signaling pathway; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of JAK-STAT cascade; positive regulation of mitotic cell cycle, embryonic; positive regulation of Notch signaling pathway; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; protein complex assembly; radial glial cell differentiation in the forebrain; regulation of fat cell differentiation; regulation of timing of neuron differentiation; smoothened signaling pathway; somatic stem cell maintenance; STAT protein nuclear translocation; telencephalon development; thymus development; transcription, DNA-dependent; trochlear nerve development
Research Articles on HES1
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Product Notes
The Human HES1 hes1 (Catalog #AAA2886983) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886983 ELISA Kit recognizes Human HES1. It is sometimes possible for the material contained within the vial of "Transcription factor HES-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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