Flow Cytometry (FC/FACS)
Rabbit anti-Mouse, Rat Fas Polyclonal Antibody | anti-Fas antibody
Anti-Fas Antibody
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
FC/FACS: 1-3ug/1x106 cells (Mouse)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Western Blot (WB)
(Figure 1. Western blot analysis of Fas using anti-Fas antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of Fas using anti-Fas antibody.)
Background: FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and it is mapped to 10q24.1. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
2. Aspinall, A. I., Pinto, A., Auer, I. A., Bridges, P., Luider, J., Dimnik, L., Patel, K. D., Jorgenson, K., Woodman, R. C. Identification of new Fas mutations in a patient with autoimmune lymphoproliferative syndrome (ALPS) and eosinophilia. Blood Cells Molec. Dis. 25: 227-238, 1999.
3. Bettinardi, A., Brugnoni, D., Quiros-Roldan, E., Malagoli, A., La Grutta, S., Correra, A., Notarangelo, L. D. Missense mutations in the Fas gene resulting in autoimmune lymphoproliferative syndrome: a molecular and immunological analysis. Blood 89: 902-909, 1997.
NCBI and Uniprot Product Information
Uniprot Description
FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine; Cell surface; Apoptosis
Cellular Component: extracellular space; neuron projection; cell surface; extracellular region; integral to membrane; CD95 death-inducing signaling complex; lipid raft; secretory granule; membrane; cell soma; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; nucleus; sarcolemma; external side of plasma membrane
Molecular Function: identical protein binding; protein binding; tumor necrosis factor receptor activity; transmembrane receptor activity; protease binding; protein complex binding; kinase binding
Biological Process: renal system process; apoptosis; positive regulation of apoptosis; response to toxin; regulation of lymphocyte differentiation; response to glucocorticoid stimulus; positive regulation of caspase activity; signal transduction; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; T cell homeostasis; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; spleen development; regulation of myeloid cell differentiation; transformed cell apoptosis; positive regulation of protein homooligomerization; negative regulation of B cell activation; negative thymic T cell selection; regulation of cell proliferation; dendrite regeneration; inflammatory cell apoptosis; B cell mediated immunity; neuron apoptosis; induction of apoptosis via death domain receptors; regulation of gene expression; immune response; gene expression; negative regulation of apoptosis
Research Articles on Fas
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Product Notes
The Fas fas (Catalog #AAA1752910) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Fas Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fas can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) FC/FACS: 1-3ug/1x106 cells (Mouse) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Fas fas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fas, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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