Canine Relaxin ELISA Kit | RLT elisa kit

Canine Relaxin ELISA Kit

Cat. Num. AAA735026

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Canine
• Synonyms: Relaxin; Canine Relaxin ELISA Kit; RLT elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This RLN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine RLN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RLT elisa kit

Principle of the Assay: RLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RLN antibody and an RLN-HRP conjugate. The assay sample and buffer are incubated together with RLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RLN concentration since RLN from samples and RLN-HRP conjugate compete for the anti-RLN antibody binding site. Since the number of sites is limited, as more sites are occupied by RLN from the sample, fewer sites are left to bind RLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RLN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RLT elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 490056
• NCBI GeneID: 6013
• Molecular Weight: 13,011 Da
• NCBI Official Full Name: relaxin
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1; preprorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Relaxin
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy; signal transduction