Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Mouse Major Basic Protein (MBP) CLIA Kit | MBP clia kit

Mouse Major Basic Protein (MBP) CLIA Kit

Gene Names
PRG2; MBP; BMPG; MBP1
Reactivity
Mouse
Synonyms
Major Basic Protein (MBP); Mouse Major Basic Protein (MBP) CLIA Kit; PRG2; BMPG; Proteoglycan 2; Bone Marrow; Natural Killer Cell Activator; Eosinophil Granule Major Basic Protein; Pregnancy-associated major basic; MBP clia kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Major Basic Protein (MBP).
No significant cross-reactivity or interference between Major Basic Protein (MBP) and analogues was observed.
Sequence Length
222
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
54.9-40,000pg/mL
Sensitivity
< 24.5pg/mL
Application
Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Major Basic Protein (MBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Major Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MBP clia kit
The microplate provided in this kit has been pre-coated with an antibody specific to Major Basic Protein (MBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Major Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Major Basic Protein (MBP) level in the sample or standard.;
Product Categories/Family for MBP clia kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
23,901 Da
NCBI Official Full Name
major basic protein
NCBI Official Synonym Full Names
proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
NCBI Official Symbol
PRG2
NCBI Official Synonym Symbols
MBP; BMPG; MBP1
NCBI Protein Information
bone marrow proteoglycan
UniProt Protein Name
Bone marrow proteoglycan
Protein Family
UniProt Gene Name
PRG2
UniProt Synonym Gene Names
MBP; BMPG; EMBP; MBP
UniProt Entry Name
PRG2_HUMAN

NCBI Description

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.

Protein type: Secreted, signal peptide; Vesicle; Secreted

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: transport vesicle; extracellular region

Molecular Function: heparin binding; carbohydrate binding

Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode

Research Articles on MBP

Similar Products

Product Notes

The Mouse MBP prg2 (Catalog #AAA2001040) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2001040 CLIA Kit recognizes Mouse MBP. It is sometimes possible for the material contained within the vial of "Major Basic Protein (MBP), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.