Typical Testing Data/Standard Curve (for reference only)
Rat c-Fos ELISA Kit | c-Fos elisa kit
Rat c-Fos ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: c-Fos ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-c-Fos antibody and an c-Fos-HRP conjugate. The assay sample and buffer are incubated together with c-Fos-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the c-Fos concentration since c-Fos from samples and c-Fos-HRP conjugate compete for the anti-c-Fos antibody binding site. Since the number of sites is limited, as more sites are occupied by c-Fos from the sample, fewer sites are left to bind c-Fos-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The c-Fos concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Uniprot Description
Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.
Protein type: Motility/polarity/chemotaxis; Oncoprotein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; cytosol; nucleus
Molecular Function: protein binding; double-stranded DNA binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; response to toxin; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; female pregnancy; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; conditioned taste aversion; transforming growth factor beta receptor signaling pathway; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; response to corticosterone stimulus; aging; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; toll-like receptor 2 signaling pathway; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to cold; toll-like receptor 9 signaling pathway; response to progesterone stimulus
Research Articles on c-Fos
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Product Notes
The Rat c-Fos fos (Catalog #AAA729725) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729725 ELISA Kit recognizes Rat c-Fos. It is sometimes possible for the material contained within the vial of "c-Fos, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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