Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
MYO3 beta Recombinant Protein | MYO3beta recombinant protein
MYO3 beta, Active
Applications
Western Blot
Synonyms
MYO3 beta; Active; myosin IIIB; MYO3beta recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Applicable Applications for MYO3beta recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for MYO3beta recombinant protein
Recombinant human MYO3beta (1-326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_138995.
MYO3beta is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain. MYO3beta contains a N-terminal kinase domain, followed by motor, neck, and tail domains. The MYO3beta gene generates a variety of splice variants that contain 1 or 2 calmodulin-binding (IQ) motifs in the neck domain and 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7-kb MYO3beta transcript in the human retina but not in a RPE cell line (1). The MYO3beta gene transcript is also detected in the kidney, intestine and testis. The MYO3beta gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment.
MYO3beta is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain. MYO3beta contains a N-terminal kinase domain, followed by motor, neck, and tail domains. The MYO3beta gene generates a variety of splice variants that contain 1 or 2 calmodulin-binding (IQ) motifs in the neck domain and 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7-kb MYO3beta transcript in the human retina but not in a RPE cell line (1). The MYO3beta gene transcript is also detected in the kidney, intestine and testis. The MYO3beta gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment.
Product Categories/Family for MYO3beta recombinant protein
References
1. Dose, A C. et al: A class III myosin expressed in the retina is a potential candidate for Bardet-Biedl syndrome. Genomics 79: 621-624, 2002.
NCBI and Uniprot Product Information
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Product Notes
The MYO3beta (Catalog #AAA517277) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MYO3 beta can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the MYO3beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYO3 beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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