Structure
Guanosine- 5'- O- diphosphate, immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose) Biochemical
Guanosine- 5'- O- diphosphate, immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose)
Synonyms
Guanosine- 5'- O- diphosphate; immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose); immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose) biochemical
Specificity
Suspension in 30 mM Na2HPO4 buffer (pH 7). Ligand density: approximately 37.2 ?mol/g of gel (dry basis). UV:λmax 252 nm/suspension in glycol.
Application Notes
Immobilized cytidine-5'-O-diphosphate (CDP-Gel, Cat. No. C 128) and uridine-5'-O-diphosphate.(Please inquire)
Properties
GDP-Gel is suitable for the affinity purification of fucosyltransferases.
Chromatography
After equilibration with 20 mM Mes-KOH, pH 6.8, containing 0.02% NaN3 and 1 mM dithioerythreitol the affinity column is loaded with the protein solution, e.g. at 1 mL/min. The column is then washed with 20 mM Mes-KOH buffer, pH 6.8, containing 50 mM NaCl, 0.02% NaN3, and 30% glycerol and subsequently with the same buffer containing 500 mM NaCl. Elution of the enzyme can be performed with the same buffer containing 1.5 M NaCl (all data according to Costa et al. 1997).
Regeneration of the gel may be achievable by incubation with 8 M urea or other chaotropic agents and subsequent washing with a suitable buffer.
Regeneration of the gel may be achievable by incubation with 8 M urea or other chaotropic agents and subsequent washing with a suitable buffer.
Preparation and Storage
GDP-Gel has sufficient stability for chromatography at ambient temperature and does not need special care
during handling or shipment. Nevertheless, for longer storage periods the gel should be kept in the refrigerator at 4 - 8°C. Storage buffer should contain 0.1% sodium azide for prevention of microbial growth.
Structure
Structure
Related Product Information for Guanosine- 5'- O- diphosphate, immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose) biochemical
Guanosine-5'-O-diphosphate (GDP) has been immobilized on a polymeric matrix via the terminal phosphate.
Similar Products
Product Notes
The Guanosine- 5'- O- diphosphate, immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose) (Catalog #AAA256264) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. Immobilized cytidine-5'-O-diphosphate (CDP-Gel, Cat. No. C 128) and uridine-5'-O-diphosphate.(Please inquire). Researchers should empirically determine the suitability of the Guanosine- 5'- O- diphosphate, immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Guanosine- 5'- O- diphosphate, immobilized on a polymeric matrix (GDP-Gel / GDP-Agarose), Biochemical" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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